NAD/NADH Assay Kit (EFND-100)

★★★★☆ 4.0 59 reviews

$509.00
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Management number 220390973 Release Date 2026/05/03 List Price $203.60 Model Number 220390973
Category

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. BioAssay Systems' EnzyFluoTM NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at ex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio. This product is for Research Use only and is not intended for diagnostic purposes.

  • For sensitive determination of NAD and NADH and evaluation of drug effects on their metabolism.
  • Method: Fluorometric assay (530/585nm). A spectrophotometer or plate reader is required, but not provided.
  • Samples: Cell, tissue extracts etc. Species: All. Detects 0.02 uM
  • Procedure: 10 min. Size: 100 tests per kit
Item Weight 1 pounds
Manufacturer BioAssay Systems
Product Dimensions 5.5 x 4 x 2 inches
Is Discontinued By Manufacturer No

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