Pyruvate Assay Kit (EPYR-100)

Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. BioAssay Systems' pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at em/ex = 585/530nm is directly proportional to pyruvate concentration in the sample. This product is for Research Use only and is not intended for diagnostic purposes.

• For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
• Method: Colorimetric (570nm), or fluorometric (530/585nm) assay. A spectrophotometer or plate reader is required, but not provided.
• Samples: Biological samples. Species: All. Detects 2 (colorimetric), 0.2 uM (Fluorometric)
• Procedure: 20 min. Size: 100 tests per kit